Extraction of bulk DNA from Thar Desert soils for optimization of PCR- DGGE based microbial community analysis
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Soil Community Analysis Using DGGE of 16S rDNA Polymerase Chain Reaction Products
ganisms increases, which provides a means to determine phylogenetic relationships and to distinguish microorSeparation of polymerase chain reaction (PCR)–amplified 16S ganisms from one another (Woese, 1987; Woese, 1992). rDNA products using denaturing gradient gel electrophoresis (DGGE) was tested as a means to study microbial community compoPresently, the rRNA genes in DNA taken directly from ...
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Ciliates play important roles as prey and predators in ecosystems. Changes in the ciliate community can affect the composition and population of microfauna and microflora in ecosystems. To investigate the structure of ciliate communities, we developed a nested PCR-DGGE method, which combines a universal eukaryotic-specific primer set in the first PCR step with a ciliate-specific primer set in t...
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